Search results for " Vasoactive Intestinal Peptide"

showing 2 items of 2 documents

Evidence for a role of inducible nitric oxide synthase in gastric relaxation of mdx mice

2006

Alterations of gastric mechanical activity have been reported in mdx mouse, animal model for Duchenne muscular dystrophy. This study examined if alterations in the vasoactive intestinal polypeptide (VIP) system are present in mdx stomach. Gastric mechanical activity was recorded in vitro as changes of endoluminal pressure and neurally or pharmacologically evoked relaxations were analysed in mdxvs normal stomach. Reverse-transcription polymerase chain reaction was used to detect inducible nitric oxide synthase (iNOS) expression. Relaxations to sodium nitroprusside in mdx stomach showed no difference in comparison with normal preparations. In normal stomach, VIP produced relaxation, which was…

Malemedicine.medical_specialtymdx mousePhysiologyMuscle RelaxationVasoactive intestinal peptideNitric Oxide Synthase Type IIStimulationDUCHENNES MUSCULAR-DYSTROPHYSettore BIO/09 - FisiologiaNitric oxidechemistry.chemical_compoundMiceOrgan Culture TechniquesInternal medicineQuinoxalinesmedicineAnimalsRNA MessengerEnzyme InhibitorsReceptorOxadiazolesbiologyEndocrine and Autonomic SystemsReverse Transcriptase Polymerase Chain ReactionStomachStomachGastroenterologySMOOTH-MUSCLE CELLSMuscle SmoothPEPTIDE RELEASENitric oxide synthaseMuscular Dystrophy DuchenneDisease Models AnimalEndocrinologymedicine.anatomical_structureNG-Nitroarginine Methyl Esterchemistrybiology.proteinMice Inbred mdxReceptors Vasoactive Intestinal PeptideSodium nitroprussideIminesmedicine.drugVasoactive Intestinal Peptide
researchProduct

NANC inhibitory neurotransmission in mouse isolated stomach: Involvement of nitric oxide, ATP and vasoactive intestinal polypeptide

2003

1. The neurotransmitters involved in NANC relaxation and their possible interactions were investigated in mouse isolated stomach, recording the motor responses as changes of endoluminal pressure from whole organ. 2. Field stimulation produced tetrodotoxin-sensitive, frequency-dependent, biphasic responses: rapid transient relaxation followed by a delayed inhibitory component. 3. The inhibitor of the synthesis of nitric oxide (NO), L-NAME, abolished the rapid relaxation and significantly reduced the slow relaxation. Apamin, blocker of Ca 2+-dependent K + channels, or ADPβS, which desensitises P 2y purinoceptors, reduced the slow relaxation to 2-8 Hz, without affecting that to 16-32 Hz or the…

NitroprussideMuscle RelaxationNANC inhibitory neurotransmitterNitric OxideSynaptic TransmissionSettore BIO/09 - FisiologiaGastric relaxationMiceAdenosine TriphosphateAdrenergic FiberChymotrypsinEnzyme InhibitorThionucleotideCholinergic FiberPharmacologyDose-Response Relationship DrugAnimalIn Vitro TechniqueMouse stomachStomachNitric Oxide DonorElectric StimulationATPVIPAdenosine DiphosphateMice Inbred C57BLNG-Nitroarginine Methyl EsterApaminReceptors Vasoactive Intestinal PeptideNitric Oxide SynthaseVasoactive Intestinal Peptide
researchProduct